fastocean fast repetition rate fluorometer (frrf)  (Chelsea Technologies Group Ltd)

Journal: PLoS ONE

Article Title: Divergence of photosynthetic strategies amongst marine diatoms

doi: 10.1371/journal.pone.0244252

Figure Lengend Snippet: Black points show individual determinations of F v ′/ F m ′ or F v /F m from FRRf measurements of cultures with PSII repair active (absence of lincomycin). Dashed orange line connects F v ′/ F m ′ measures taken immediately after exposure to HL (1200 μmol photons m -2 s -1 ) over 0–120 min or recovery light (15 μmol photons m -2 s -1 ) at 150 and 180 min, influenced by combined effects of non-photochemical quenching induction and net photo-inactivation (if any). Solid green line connects F v /F m taken after 10 min of subsequent dark for HL time points, to allow relaxation of non-photochemical quenching, or taken immediately during the recovery light period. These points were used to fit the Kok model of PSII photo-inactivation countered by repair. Note the different patterns and amplitudes of short-term (10 min) relaxation of non-photochemical quenching, among the species (black dots). The dotted red line shows F v /F m data from separate lincomycin treated cultures to show the underlying photo-inactivation in the absence of counteracting repair.

Article Snippet: Photophysiological assessment was performed using a FastOcean Fast Repetition Rate fluorometer (FRRf; S/N: 12-8679-007) fitted with a FastACT laboratory base unit (Chelsea Technologies Group Ltd, London, U.K), largely as per previous protocols [ , ].

Techniques:

Journal: PLoS ONE

Article Title: Divergence of photosynthetic strategies amongst marine diatoms

doi: 10.1371/journal.pone.0244252

Figure Lengend Snippet: (A) Mean values of YNPQ vs. the rate constant for PSII repair, k REC , over 120 min HL exposure showing nonphotochemical quenching measured immediately after light exposure (larger symbols) or after 10 min dark FRRf incubation (smaller symbols) for T . weissflogii (Tw, blue triangles), T . oceanica (To, red diamonds) and T . pseudonana (Tp, black squares). (B) The amplitude of YNPQ relaxation (also green arrows in (A)). Error bars show standard errors of the estimates for 3 or 4 independent biological replicates.

Article Snippet: Photophysiological assessment was performed using a FastOcean Fast Repetition Rate fluorometer (FRRf; S/N: 12-8679-007) fitted with a FastACT laboratory base unit (Chelsea Technologies Group Ltd, London, U.K), largely as per previous protocols [ , ].

Techniques: Incubation